Abstract
For tissue engineering, it is very important to construct cell patterns and immobilize patterned cells inside certain structures. In this paper, we present a novel method of forming line pattern of yeast cells by dielectrophoresis (DEP) and immobilizing patterned cells by photo-crosslinkable resin. Microstructure embedding patterned yeast cells is on-chip fabricated. Several microelectrodes are fabricated by Indium Tin Oxides (ITO) for cell patterning by DEP. The two kinds of DEP responses of yeast cell (W303) and the experimental parameters are experimentally confirmed. Based on negative DEP phenomenon, cell traps generated by microelectrode are demonstrated. A cell trap matrix is fabricated and high speed cell pattering is performed. The experimental results show that the cell line patterns which contain hundreds of yeast cells can be formed by DEP within 1 second. On-chip fabrication method for arbitrary shapes of microstructures based on Poly Ethylene Glycol Diacrylate (PEG-DA) is reported. With the cell patterning by DEP and immobilizing by on-chip fabrication, microstructures embedding 3 lines of yeast cell are directly fabricated inside microfluidic chips, in PEG-DA and NaC1 solution.
