2A1-S05 Development of temperature-responsive cell culture surface by using PDMS substrate and characterization of the surface(Bio Assembler for 3D Cellular System Innovation)

Abstract

Stretchable temperature-responsive cell culture surfaces was newly prepared by grafting poly(N-isopropylacrylamide) (PIPAAm) on polydimethylsiloxane (PDMS) (PIPAAm-PDMS). The PIPAAm-PDMS at non-stretched state showed temperature-induced cell attachment and detachment properties similar to conventional PIPAAm-TCPS, while that at stretched state did temperature-induced cell attachment but slower detachment properties than that at non-stretched state. As expected, such slow detachment is due to decrease of PIPAAm thickness and PIPAAm graft density as well as increase of hydrophobic property induced by stretching procedure. This result also suggests that hydrophobicity of PIPAAm-PDMS surfaces is appropriately adjusted to target cells to be cultured by only stretching the surfaces.