Abstract
We proposed a microfluidic chip having fluorescent oxygen sensor for measurement of oxygen consumption rate (OCR) of oocyte. The chip is made of Polydimethylsiloxane (PDMS), which is oxygen permeable, and the upper part is open to make introduction and collection of oocyte easy. Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)2Cl2) is used as fluorescence oxygen indicator. Ru(bpy)2Cl2 is embedded into hydrogel made of Polyethyleneglycol diacrylate (PEG-DA). Stripe-shape oxygen sensor is fabricated by photolithography and replica molding method. Oxygen consumption rate was calculated based on spherical diffusion equation. This method achieve simultaneous measurement of two-dimensional distribution of OCR by acquiring the fluorescent image. We demonstrated measurement of OCR of the single mice oocytes (Average OCR: 0.17 ± 0.02 fmol/s).
