The single cell analysis has attracted much attention to reveal the detailed and localized biological information, which is not obtained by the conventional group cell analysis due to the statistical assay, on individual cells. To analyze the detailed and localized properties of single cells, local environmental control technique, which is the technique to change and sense the local environment around single cells, is effective. Therefore, local environmental control technology for single cell analysis is desired and being established. In particular, a system that operates with stability is important. To build the system which can achieve high speed (high time resolution) and prolonged analysis is useful for an understanding of various life processes. For example, biological molecular motor is a good candidate for analysis. In this study, we try to develop a real time and long time observation system that can changing and keeping local environment condition stably. And then we apply this system for analyze the one kind of biological molecular motor, "bacteria flagella motor", which rotational principle is largely unknown.
