1P1-P05 Development of Micro Vacuum Chuck for Tensile Test of Three Dimensional Multilayered Tissues

Abstract

The mechanical properties are indispensable parameter for evaluate the tissue structures. In this report, we developed a micro vacuum chuck which could fix the three-dimensional multilayered tissue to tensile test system without breakage of sample. The three-dimensional multilayered tissues were fabricated with technique of layer-by-layer (LbL) nanofilm coatings. By using the micro vacuum chuck, we succeeded to measure tensile force of the three-dimensional multilayered tissues. In the result, the maximum tensile force was about 626 μN (strain: 1.9). When maximum force occurred, the sample detached from the chuck. The profile of force-strain curve was similar to that of tendon or the ligament. This result indicates that the three-dimensional multilayered tissue has mechanical properties which are same to living tissues.