Abstract
Piezoelectric inkjet printer has been applied into the bio-field to deposit proteins and living cells due to its high speed printing capability and high resolution patterning of small bio-ink droplets. Recently, it is also utilized to deposit single cells on arbitrary pattern in order to isolate single cells for further analysis or for high resolution biofabrication application. Better spatial control of cell patterning possibly allowed fabrication of a more delicate structure. In this research, shutter system was developed and combined with an automatic single cell printer based on piezoelectric inkjet printer to deposit single cells on collagen substrate for biofabrication application.