This study successfully fabricated a dimpled cellular tissue by using the interface between the gas and liquid of air bubbles generated on cell suspension. The bubbles were structured with a hexagonal pattern and its interface was named as structured interface. Ten million of cells were once formed with dimpled surface within 2-h cultivation with the structured interface. The cells were excluded from the structured interface and accumulated into the gap among bubbles. This study called the formation and accumulation of cells by the structured interface "inverted-scaffold effect." The dimpled cellular tissue might be a possible solution to improve the cellular viability of thick tissue in the initial stage of cell culture.
