遊走能が亢進したがん細胞の選択回収マイクロ流路デバイス

抄録

We propose a micro-channel device to collect cancer cells with high migrating capability, which could cause metastasis. In order to collect cells attached on extracellular matrix, focal adhesions between cells and extracellular matrix should be broken. The desmosomes can be mechanically broken by using extension of cell culture surface. For this purpose, the device locally has a balloon under a cell culture surface. The extension rate of the cell culture surface is 40% with 300 μL water. Using the device, we could detach the high-migration cell, which has net displacement of 150 μm and total path of 250 μm, and collect it by the flow of cultural medium.