Fundamental Study of On-chip Micro-Heater and Sensor for Local Temperature Control of Cells

Abstract

For the present study, local temperature controlled micro-fluidic channel to culture cells was fabricated and evaluated. Inverted microscope is widely used to observed cells such-as time-lapse images for long time. On-chip transparent micro-electrode (ITO) as heater is promising method to observe cells with local temperature control. Thermo-sensitive gel was also employed to immobilize and to obtain positioning of cells with temperature control by fabricated fine Au sensor to prevent from excessive temperature rise. This study has potential to contribute to study temperature response of cells and provide new cellular response and functions.