光環境センシング

－細胞培養状態のマルチモーダル計測－

抄録

This paper reports the measurement of the multimodal environmental condition such as pH and calcium ion concentration during the cell culture using fluorescence microsensors fabricated on the glass substrate. These fluorescence microsensors are coated with gelatin, which is used as the extracellular matrix, for the culture of the osteoblast cells on the gelatin surface. The fluorescence microsensors consist of hydrophilic photo-crosslinkable resin and three fluorescence indicators. Fura-2, FITC, and Rhodamine B are used for measurement of calcium ion concentration, pH, and temperature, respectively. The sensitivities of each fluorescence indicator are compensated by using fluorescence intensity of other fluorescence indicators. We measured local variation of calcium ion concentration and pH during conversion of octacalcium phosphate (OCP) to hydroxyapatite (HA) using fluorescence microsensors. We also demonstrated the culture of the osteoblast cell on the fluorescence microsensors.