The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)
Online ISSN : 2424-3124
2018
Session ID : 1P1-K09
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Pressure Homogenized Photooxidative Cell Membrane Perforating System for Tissue Engineering
*Takashi Kei SAITOTomoyuki SAKAKIBARATakahiro NAKAMURATakayoshi OHBA
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Abstract

Our photochemical cell membrane perforation method is new approach for mass cell processing, eg. cell therapy or regenerative medicine. The method requires homogeneous control of cell attaching pressure of nano brush shaped cell membrane perforator which contains photosensitizer, but it was hard because almost the cell size are micrometer scale and its structure is weak. We made the fluid-packed perforator to homogenize the applying pressure, based on Pascal's principle. This perforator has flexible surface which can adopt variations of cell layer on culture dishes. In this research, we developed the extended system for tissues and will report a trial effort on mouse tissues.

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© 2018 The Japan Society of Mechanical Engineers
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