Extension of Bidirectional Electroosmotic Pump to Parallel Single-Cell Manipulation

Abstract

The objective of this research is to develop a versatile cell manipulation system for patterning single cells with high accuracy in a parallel format. For this purpose, we fabricated a monolithic microdevice incorporating a micronozzle array and pumps formed from ITO electrodes. This pumping mechanism is based on a DC-biased AC electroosmotic (ACEO) flow. We used this pump and generated a flow around the electrodes, and the flow transported a fluid containing particles or cells.