周波数変調式力センサを用いた細胞のオンチップ動的粘弾性計測の検討

抄録

This paper proposes the analytical results of frequency modulated force measurement in microfluidic chip to characterize the single cell with their mechanical properties. Like the configuration of atomic force microscopy, we have developed the microfluidic devices having mechanical force probe to perform the mechanical characterization of single cell with high throughput. Contrary to the previously reported devices, we considered the dynamically oscillating force sensor alternative to the one using static deflection of the microbeam structures. Since the method utilizes the amplitude and frequency shift independently, both the conservative and the dissipative force are obtained at the same time. The main topic of this report is to consider the measurement accuracy and throughput. Our previous research enables the high speed actuation mechanism of on-chip force probe, the displacement sensor with high measurement speed. On the other hand, the theoretical background on the dynamic cell characterization was not definitely explained and examined. Therefore, we derived the theoretical requirements. As a result, we found that the noise level of the laser displacement sensor should be further improved for the practical use of single cell characterization. This analysis should be the fundamental for the upcoming cell characterization devices.