光環境センサの高速細胞導入のための波長特異的レーザ加熱

抄録

In this paper, we propose the manipulation and cell injection of the fluorescence microsensor using multiple wavelength lights. The sensor made of 1 μm polystyrene particle containing Rhodamine B and infrared (IR: 808 nm) absorbing dye. The polystyrene particle can be manipulated by 1064 nm in water since the refractive index of the polystyrene is 1.6 (refractive index of water: 1.3). Rhodamine B is temperature sensitive fluorescence dye (emission wavelength: 560 nm). IR absorbing dye absorbs 808 nm light but don’t absorb 1064 nm laser. Transport of the fluorescence microsensor with optical tweezers by 1064 nm laser. After transport to the membrane of target cell, the fluorescence microsensor is heated by 808 nm and injected into the cell. Temperature increase at 808 nm (40 mW) illumination was approximately 27℃ for 60 seconds. We demonstrate manipulation and injection of the microsensor to the MDCK cell using wavelength-specific laser heating. From these results, we confirmed the effectiveness of our proposed method using multiple wavelength lights.