Study on Fabrication of Cellar Three-dimensional Liver Model Using Self-organization by Magnetic force

Abstract

One of the important tasks of research in the field of regenerative medicine is to construct three-dimensional cellular tissues including vascular structures in vitro. In this research, we propose a method to form a tube structure that can supply nutrients and oxygen using magnetic fiber and magnet tweezers for three dimensional culture of hepatocytes with gel as a scaffold. Magnetic fiber was placed on the plane, magnetism was generated in the magnet tweeter, and the fiber was automatically guided to the desired shape, and the gel as the scaffold of the cell was poured and solidified. After repeating this operation, the hepatic lobule-like three-dimensional structure having a tubular structure was constructed by removing the fiber.