Internal condition measuring for “Organomachines”

Abstract

This paper describes in-situ measuring method of internal condition for a skeletal muscle tissue. Skeletal muscle tissue with hydrogel scaffold changes it internal condition during the cultivation in the real time. Skeletal muscle tissue with hydrogel scaffold are placed in a tensile platform, to measure tension using a force sensor mounted on a tensile platform while uniaxially moving. Since a tensile platform is installed in CO₂ incubator including microscope, Tension and internal condition of skeletal muscle tissue are observed for a long term according to the maturation difference. In the formation of the skeletal muscle tissues, we 3D printed myoblast-laden hydrogel and cultured them to construct a skeletal muscle tissue on support substrate of the tensile platform. Further we confirmed that the skeletal muscle tissue can observe internal oxygen concentration by microcapsule sensor.