Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : June 05, 2019 - June 08, 2019
This paper reports an improved method for applying the mechanical stress on cells in a micro channel. It is reported that cancer cells can respond different characterization to the confined extracellular environment in order to cross basement membranes and connective tissues. However, it is unknown that how cancer cells respond and show the adaption to confined environment. In terms of these issues, we design and develop a one-layer microfluidic device to simulate a confined extracellular environment, and it can apply sequential mechanical stress on a number of cells to study the cell characterization. During experiments, normal and cancer cells suffer 20 mechanical stresses in micro channel respectively, and a high-speed camera under microscope collects the information of passing time. According to the passing times of normal cells and cancer cells, the preliminary results indicate that the characterization of cancer cells is different from normal cells, and they show more adaption during suffering multiple mechanical stresses.
