細胞の均一播種に資するバイオ実験者動作の特徴分析

抄録

In this study, we analyzed experimenters’motional characteristics that lead to uniform cell seeding. The motion to seed cell was measured using a motion capture system for three novices who were taught to execute stirring by ”a rotational movement” of a multi-well plate and four experients who were asked to seed as usual. Skill indices were defined as a feature of the seeding motion. Cell uniformity was evaluated as an averaged intensity variation from binarized microscope cell images. The correlation analysis between each skill index and uniformity index showed that the stirring movement of the plate was strongly related to uniformity of the seeded cell. Significant difference between the novice group and the experient group was observed in uniformity index and several skill indices in stirring process. These results indicated that teaching rotational stirring to a novice worked to guarantee uniform cell seeding to a certain extent.