Development of micro-channel device for isolation and observation of Arabidopsis thaliana root

Abstract

Micro-channel devices for cell manipulation are being actively developed, but there are very few reports on plants compared to animal cells including human cells. This time, we will introduce root isolation and observation methods for Arabidopsis thaliana roots using a micro-channel device. Specifically, we optimized the height and width of the channel through which only 1 of Arabidopsis thaliana root can pass. Since Arabidopsis thaliana is a model organism in plant research, it was targeted this time. In the future, based on the findings obtained in this study, we would like to analyze the behavior of individual roots and clarify the correlation with repellent substances by utilizing the characteristics of microchannel devices.