Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : June 01, 2022 - June 04, 2022
In this work, an FPGA-based impedance cytometry is proposed to characterize the morphology and interior of single cells. When the detection frequency is sufficient to penetrate the cell membrane (6 MHz), uneven intracellular component distribution can alter morphology of impedance pulses. When the frequency is below 1 MHz (500 kHz), the morphology of low-frequency impedance pulses is related to the shape of single cells. Additionally, the magnitude of impedance signals (500 kHz and 6 MHz) gets the dependence on the cell volume and interior biomass, respectively. The detection method is label-free and high-throughput, and successfully demonstrates the ability to track changes in biophysical properties
