動的環境下における単一プロトプラスト高速分取システム

抄録

Arabidopsis is a model plant for studying the mechanism of plant growth and development. Many studies on Arabidopsis requires the analysis of the root tip. Previously, biologists always cut the root tip and put them in enzyme solution to isolate the protoplast and analyze the RNA or protein of the cells. But this method has a disadvantage. The location information of protoplasts is lost, and more advanced gene expression analysis cannot be performed. In this research, we propose a single cell level protoplast pickup system to isolate cells from the root tip under dynamic environment. Specifically, the system integrates an optical inverted microscope, a camera for image analysis, a micromanipulator and piezo pump for single protoplast pickup and a chip for root immobilization and observation. Using this system we have achieved automated 3D pipette tip calibration and automated single protoplast pickup.