Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : June 28, 2023 - July 01, 2023
This paper describes a method for integrating non-contact fluorescence measurement functionality into multilayered metal micro-particles that can be manipulated by optical tweezers. The fluorescence intensity of the fluorescent dyes depends on the environment. Tt is necessary to integrate a measurement sensor and a reference sensor to perform absolute measurements. However, even with microfabrication techniques such as photolithography, it is difficult to integrate a measurement sensor and a reference sensor onto a sensor with a diameter of several micrometers. In this study, a microsensor that could be manipulated by optical tweezers by coating the surface of crystalline metal titanium oxide micro-particles with a layer of amorphous titanium oxide was fabricated. The sensor layer and the reference layer were integrated into one micro-particle by modifying the surface of both the metal and amorphous layers with pH-sensitive fluorescent dye. Observation of the two fluorescent dye layers and manipulation by optical tweezers in the fabricated approximately 2 μm diameter fluorescent sensor were successfully demonstrated.
