光操作が可能なリファレンス機能を統合したメタル光環境マイクロセンサ

抄録

This paper describes a method for integrating non-contact fluorescence measurement functionality into multilayered metal micro-particles that can be manipulated by optical tweezers. The fluorescence intensity of the fluorescent dyes depends on the environment. Tt is necessary to integrate a measurement sensor and a reference sensor to perform absolute measurements. However, even with microfabrication techniques such as photolithography, it is difficult to integrate a measurement sensor and a reference sensor onto a sensor with a diameter of several micrometers. In this study, a microsensor that could be manipulated by optical tweezers by coating the surface of crystalline metal titanium oxide micro-particles with a layer of amorphous titanium oxide was fabricated. The sensor layer and the reference layer were integrated into one micro-particle by modifying the surface of both the metal and amorphous layers with pH-sensitive fluorescent dye. Observation of the two fluorescent dye layers and manipulation by optical tweezers in the fabricated approximately 2 μm diameter fluorescent sensor were successfully demonstrated.