抄録
Production of artificial organs utilized in vivo and in vitro is the main topic of tissue engineering. However the method of designing their structures and the optimization of their specifications in terms of physiological functions are still not established. Metabolism of living cells that affects physiological functions is the most important data for this purpose. In this study, glucose consumption rate of HepG2 cell line at different temperature and in glucose concentration medium was measured by using the noble system that allows the measurement in steady mass transport condition with small number of cells, c.a. 10^5 cells. The results provided the several parameters of Michaelis-Menten model at different temperature.
