Process of biodiesel fuel (BDF) production from microalgae desmodesmus is analyzed from the view point of energy consumption and CO_2 emission. The process consists of filtration, drying, extraction, and esterification. Because filtration deals with huge amount of water comparing with the weight of harvested microalgae, improvement of efficiency in this process is mandatory for total system efficiency. To reduce the energy consumption and CO_2 emission during the drying and esterification processes, it is also important to develop new single process to synthesis directly BDF from wet algae.