抄録
This research was carried out to discuss the possibility of the new cryopreservation method without giving the damage to cell. This method was performed by giving the micro electric current to plant cell. Jerusalem Artichoke and Saxifraga stolonifera were mainly used for the tissue and protoplast of plant. As a result, this method could make the ice crystal formed finer and make the temperature at the release of supercooling lower in the range of 3℃〜12℃. To investigate its mechanism, the cytoplasm was measured by a fluorescence pH-ratiometry. It was clarified that though cytoplasm became acidified with the progress of cooling, it shifted to be alkalified by the addition of micro current.
