凍害防御剤の卵内導入によるメダカ卵の凍結制御

抄録

For purpose of cryopreservation of fish eggs, we have done freezing experiments of medaka eggs using liquid meniscus. But, we have not been able to confirm hatching of eggs after intracellular freezing. In order to reduce damage due to intracellular freezing, two capillaries were used to extract the internal solution and inject the cryoprotectant into eggs. From the measurements of egg freezing temperature, melting temperature and solidification latent heat by using a differential scanning calorimeter(DSC), it was possible to confirm clear decrease in freezing temperature and release of latent heat.