細胞の冷温保存におけるアルゴンガス加圧溶解による保存温度に対する保護効果と至適圧力の検討

抄録

The protective effect on storage temperature and the optimal pressure by pressurized dissolution of argon gas in cold storage on a rat heart striated myocytes monolayer were investigated. The cell monolayers incubated in a culture dish for 24 h were prepared as test samples. The samples were pressurized in 0, 0.7, 0.8, 0.9, or 1.0 MPa in argon gas, and then preserved at 4 and 6°C for 24 h. The pressure-dependent cell viability was evaluated using the water-soluble tetrazolium salts assay. The results showed that protective effect in 0.8 MPa was highest for each temperature, and the protective effect at 6°C was higher than that at 4°C.