抄録
Ascogregarina sp., the nonpathogenic parasite of Aedes sp. is expected to have the potentiality to be used as a gene vector. A comprehensive study was conducted to develop the method of extraction of sporozoites of both Ascogregarina taiwanens and As. culicis from their oocysts using the mechanical, chemical and biological method. The result revealed that the biological method enables the release of about 98% active sporozoite. The sporozoites are yielded from any stage of larval midgut of Aedes species within 45 minutes to 2 hours of the ingestion. Moreover, Ascogregarina sp. showed no host specificity to be released from the oocysts. We have also found that the sporozoites are released even in the midgut of late instar larvae and attained its matured oocyst.
