犬小腸の体外保存ならびに移植における平滑筋筋電図所見について

抄録

Extracorporal preservation and heterotrophic allotransplantation of small bowels in adult dogs were carried out.
1. Experiment of preservation
After flushing out of blood from vessels by 5% low molecular dextran, small bowels were preserved at -5°C, 0°C, 4°C, 10°C and 23°C respectively, wrapping in gauze humidified with autoplasma.
The electromyograms of the preserved bowels were recorded at regular time intervals, in order to find out the maximum period of preservation. The E. M. G's which were recorded from those preserved at 4°C kept almost same activities until the 14th days after preparation.
2. Experimental transplantation
The isolated terminal ileum of 30 cm was transplanted into the abdominal cavity, performed as the Thiry-Vella fistula, or at the place of excised cervical esophagus.
Imuran, 6MP and M. M. C with dexamethasone were used in allotransplantation as immunosuppresive agents. A dog with the Thiry-Vella fistula survived for 79 days and it was the longest survival of the allograft. However, the dog with cervical transplantation survived for 35 days at maximum.
Several changes were observed in E. M. G's of these two groups. These changes coincided with the time of rejection crisis at 2 weeks after operation.
Small bowels transplanted into cervical esophagus revealed to be nearly normal in view of E. M. G's which were recorded 4 months after operation, while in the cases of the Thiry-Vella fistula bowel activity could not recorded.
In the Thiry-Vella fistula group without immunosuppresive agents transplanted bowels were rejected at an earlier period.