Abstract
This study investigated the influences of vitamin D restriction on alkaline phosphatase (ALP) activity in rats fed a high-fat diet. Male Sprague-Dawley rats (11 weeks old) were divided into four groups: those fed a basic control diet (C) , those fed a basic control diet with vitamin D restriction (DR) , those fed a high-fat diet (F) , and those fed a high-fat diet with vitamin D restriction (FDR) . At 28 days after starting the experimental diet, ALP-specific activity in the femur had decreased significantly in the DR group relative to the C group, and also in the FDR group relative to the F group. Furthermore, the ALP-specific activity in the duodenum had decreased significantly in the FDR group relative to the F group. As it has been suggested previously that intestinal ALP detoxifies a variety of bacterial toxins including LPS and prevents metabolic syndrome, our findings indicate that restriction of vitamin D in a high-fat diet may influence gut homeostasis by decreasing the level of ALP activity.
