Abstract
To investigate the riboflavin biosynthesis of Bacillus natto, the total contents of riboflavin in bacillus and ‘natto’ were measured by lumiflavin fluorometric method, and also the contents of free riboflavin and of nucleotide forms (FAD and FMN) were separately measured.
The results obtained are as follows:
1) The riboflavin content of Bacillus natto is found to be 6.8-8.4μg/g, and this value is higher than that of muscle.
2) It might be concluded that Bacillus natto contained only FAD and FMN, and no free riboflavin.
3) In ‘natto’, the content of FAD is found to be the largest of these three forms of riboflavin and its derivatives, and as compared with the distribution of these riboflavin derivatives in soy bean, it might be transformed the free riboflavin in soy-bean to FAD form.
