The recovery of methionine from acid-hydrolysate of animal-foods is higher than that of plant-foods. It has been recognized that the error of recovery was caused by carbohydrates present in foods.
But the relation between the recovery and, kinds and amounts of carbohydrate in foods is not known clearly. On this account, some model-experiments, in which carbo-hydrates were added to casein in several rates, were carried out. In those experiments, hydrolysis was carried out with 2N-HCI for 5hrs. under 15 Ib..
In results, the relation of methionine recovery (y) and the rate of carbohydrate to the casein-nitrogen (x) was expressed as following: y=100.02-0.26 (x) on the case of potato-starch, y=99.24-0.44x on dextrin, and y=96.84-0.52x on glucose. But, fibre has no effects on the methionine recovery under such conditions.

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Flaked germs of wheat, both raw and roasted, contained about 250mg% of total glutathione (G) and about 100mg% of reduced G. The losses of both forms of G. during storage at rather unfavorable conditions were more than 40%, but the losses were smaller in the case of the roasted germs than those of raw ones.
Rice germs and barley germs contained less amount of G. in comparison with wheat germs, the losses of G. during storage were about 10-80%.
The amount of G. of both forms in the wheat flour was higher in the flour of high gluten, fresh or not breached, and they contained about 7mg% of the reduced form and about 30mg% of the total G. And another or stored flours contained about 3mg% of the former and about 20mg% of the latter.

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1. Fresh green vegetables preliminary dipped in the solutions of antibiotics and then stored in ordinary room could keep its freshness and also retained generally higher ascorbic acid content in comparison with those treated with water.
2. When stored the antibiotics treated vegetables at 5°C, its effects were only scarcely recognized in appearance, while the retention of ascorbic acid was also higher than that of control.

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Fundamental investigations on the effects of different kinds of fungicide on the chemical composition of rice kernels, Hokuriku No. 14, were made. As to the contents of general constituents of rice kernels, the effects of fungicides were hardly noticed. Copper, one of the main constituents of fungicides, was found in all plots, but quantitative difference among them could not detect. Mercury was unable to determine with spectrum analysis in this test, but Pb, Sn, Mg and Ag were found particularly in the fungicided plots. These metalic elements, which were conjectured to relating with impurities of fungicides, might be presumed to translocate into the rice kernels. The contents of thiamine and riboflavin were remarkably affected by fungicides; thiamine was increased and riboflavin was decreased in fungicided plots.

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Fundamental investigations on the riboflavin contents of rice kernels were made under different conditions of fertilizers at three locations in Miyagi Prefecture, using two varieties of rice plant-Norin No. 21 and No. 24.
As to the yield of rice, the effct of nitrogen was notable, but its effect was varied with the quality of soil. Phosphorus was seemed to brought greater effect in the biosynthesis of riboflavin than the other conditions. Therefore, it was assumed that phosphorus has an important roll in the biosynthesis of riboflavin, although its tendency was varied with the quality of soil.
In the case of clayey-loam, it was difficult to observe the difference among the effect of the varieties to the biosynthesis of riboflavin and of fertilizers, however, in the case of peat-soil, varietal difference was remarkable.

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In order to know the effect of exercise on calcium absorption, a 3-week's study was made with 9 children, every about 10 years old.
It was shown that calcium absorption was increased during sports activity period as much as during glycine administration period, which is well known to increase the rate of calcium absorption.
Therefore it has been proved that sports activities, as well as glycine administration, make the calcium absorption to raise.

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The weight of body and several organs of under-nourished rats, which were administered little mineral elements, were significantly less than those of the well-fed rats.
Phosphorus and calcium content in bone, large and small intestine wall, stomach wall, liver, spleen and heart of the under nourished rats were also diminished, while sodium and potassium content did not. Phosphorus and calcium content in kidney and muscle were relatively greater than those of the other organs in the case of under-nourished rats, while not so different absolutely in most cases.
Iron and copper contents were also analysed.

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Each under and well nourished rats increased in their body weight by vitamin D₂ administration, and the phosphorus, calcium, iron and copper content increased in bone and liver. The effect of vitamin D₂ was clearly observed in the under-nourished rats, except potassium content in bone which was decreased contraversely.
The calcium content in bone, liver, kidney and other organs of both group of rats increased remarkably by calcium administration.
The copper content increased except the wall of digestive organs, the iron content also increased inbone and liver but in the other organs of under-nourished rats sometimes decreased by vitamin D₂ administration.

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Investigations are made on the physical development and nutritional intake of primary schoolchildren with angular stomatids in a farm village in Miyagi Prefecture.
Schoolchildren with angular stomatitis are a little inferior to those without the disease in development of height, weight and chest for a year, and their food intake tends to be in amount of carbohydrates and shows heavy deficiency in animal protein, vitamin B₂, nicotinic acid and calcium. This is the general tendency on the alimental habits of farmvillage inhabitant.

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Studies are made on the physical development of primary schoolchildren (sixth grade) and middle school students (third grade) with angular stomatitis in Sendai City.
Schoolchildren with angular stomatitis are inferior to normal children of the same age in particularly in development of height rather than in weight and chest. Thus it is considered that qualitative disharmony in alimental habits is important in the occurrence of angular stomatitis and influences unfavorably upon the development of height.

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To investigate the riboflavin biosynthesis of Bacillus natto, the total contents of riboflavin in bacillus and ‘natto’ were measured by lumiflavin fluorometric method, and also the contents of free riboflavin and of nucleotide forms (FAD and FMN) were separately measured.
The results obtained are as follows:
1) The riboflavin content of Bacillus natto is found to be 6.8-8.4μg/g, and this value is higher than that of muscle.
2) It might be concluded that Bacillus natto contained only FAD and FMN, and no free riboflavin.
3) In ‘natto’, the content of FAD is found to be the largest of these three forms of riboflavin and its derivatives, and as compared with the distribution of these riboflavin derivatives in soy bean, it might be transformed the free riboflavin in soy-bean to FAD form.

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The loss of l-ascorbic acid while cooking was investigated with the cuisines of Japanese and European styles, according to the normal menu at the monastery to which the author is belonging to.
The average amounts of l-ascorbic acid contained in the raw materials prepared for these two styles of cuisines were found to be 82mg. per day for European and 54mg. for Japanese, and the loss of l-ascorbic acid while cooking was 29% for the former and 36% for the latter.
As the l-ascorbic acid content of natural foodstuffs was found to be somewhat smaller than that cited in the Food Composition Tables, the loss of this vitamin attains to 40% for European and 45% for Japanese cuisines on the base of the reference data.

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