Abstract
The method of thin layer chromatography was investigated as a possible means of separating and determining free sugars.
The best results were obtained with cellulose powder (Avicel SF) as carrier and ethyl acetate, pyridine, acetic acid, water (5: 5: 1: 3) as mobile solvent.
Sugars were determined by the new quantitative method of measuring the color produced by reaction with 0.1% gallic acid at 550mμ in conc. sulfuric acid.
The method of TLC-gallic acid was comparable to Wolfrom's method.
