Abstract
The authors investigated the effect for formation of coenzyme form of vitamin B group or coenzyme Q values by using rats bred with gluten diet. Moreover, oxidative phosphorilation was measured to follow the correlation of vitamin B12. The male rats of Wistarstrain, weighing about 30g, were fed with 1μg/rat/day of vitamin B12 and this feeding was continued for 60 days. Livers were isolated and submitted to the following measurements.
The vitamin B12 contents were measured by microbioassay using L. leichmannii, total B2 by lumiflavin method, FAD and FMN, FR by paperchromatography and lumiflavin method, NAD using Alcohol dehydrogenase, Co-Q by photometric method at 275nm, oxidative phosphorilation by oximeter with mitochondria fraction.
The following results were obtained.
(1) Vitamin B12 and Co-Q values were clearly increased compared with control group.
(2) P/O ratio of a liver mitochondria fraction from vitamin B12-administered rats was confirmed higher than that of control.
From these results, the authors suggested the role of vitamin B12 on a rat liver mitochondria energy formation system.
