One g of parsley in 30ml of distilled water was treated with a 20KHz sonic oscillator (Toyo N-50-4) for 10min. By this treatment, 90% of the bacteria naturally contaminating on unwashed parsley were killed. However, a similar result was obtained by hand-washing for only 1min. The hand-washing was thus more effective than the sonication in removing bacteria from parsley.
Fifty g of parsley in 1 liter of distilled water were treated with a 50KHz sonic oscillator (Bransonic 12) for 10min. The survivals of bacteria on the parsley and in the washing medium were approximately 25% and 45%, respectively. The bactericidal activity of this apparatus was thus inferior to that of the above one. When parsley was furiously washed by hand in 1 liter of distilled water for 5min, all of the bacteria on the parsley were left alive. The distribution of the bacteria was approximately 30% on the parsley and 70% in the washing medium. In this case, therefore, the sonication was more efficacious than the hand-washing.
Twenty g of parsley in a glass beaker (1 liter) was washing by hand in running tap-water for 1min. Most of the bacteria was removed by this washing. Any other methods of washing used in the present study were not so effective as this.

View full abstract

The authors investigated the effect for formation of coenzyme form of vitamin B group or coenzyme Q values by using rats bred with gluten diet. Moreover, oxidative phosphorilation was measured to follow the correlation of vitamin B₁₂. The male rats of Wistarstrain, weighing about 30g, were fed with 1μg/rat/day of vitamin B₁₂ and this feeding was continued for 60 days. Livers were isolated and submitted to the following measurements.
The vitamin B₁₂ contents were measured by microbioassay using L. leichmannii, total B₂ by lumiflavin method, FAD and FMN, FR by paperchromatography and lumiflavin method, NAD using Alcohol dehydrogenase, Co-Q by photometric method at 275nm, oxidative phosphorilation by oximeter with mitochondria fraction.
The following results were obtained.
(1) Vitamin B₁₂ and Co-Q values were clearly increased compared with control group.
(2) P/O ratio of a liver mitochondria fraction from vitamin B₁₂-administered rats was confirmed higher than that of control.
From these results, the authors suggested the role of vitamin B₁₂ on a rat liver mitochondria energy formation system.

View full abstract

[in Japanese]

View full abstract

Most observes have found that circulating red cell volume deduced from measurement of plasma volume and venous hematocrit demonstrates a higher value than that measured by direct method. The most reasonable explanation for this phenomenon is that the cell percentage of the blood in small vessels is lower than that in the large arteries and veins.
When both plasma and cell volumes are determined simultaneously, one obtains a true measure of total blood volume and can calculate the overall cell percentage. If the ratio of overall cell percentage/large vessel cell percentage, or F_cells value is constant in large physiological variation of rats, one can be safely used to deduce blood volume from a large vessel hematocrit and an estimation of plasma volume.
Normal and splenectomized rats with wide range of body weight and with different levels of protein in the diets were used for the experiment. With the use of ⁵¹ Cr and T-1824, red cell and plasma volumes are measured simultaneously in the rat. Red cell and plasma volumes per unit body weight are decreased in accordance with the increase in body weight, and they are also reduced by feeding a low protein diet.
F_cells value of the rats shows neither significant difference with body weight, nor nutritional status, and is unchanged by splenectomy.

View full abstract

Oubu-shi, Aichi Oligosaccharides were extracted from soybean with 80% ethanol and an automated assay was carried out with them by employing the partition chromatography on a stmngly basic anion exchanger. 0.05M borate (pH 7.5) and 0.13M borate (pH 7.5) were used as the bufferes for development. A standard mixture of authentic oligosaccharides gave a stable reproducibility in this method; the coefficient of correlation of the reproducibility was 0.98 or higher. The analytical data obtained with the soybean oligosac-charides showed a standard deviation of less than 1.5%.

View full abstract

In order to clarify the relation between the antioxidant activities and the tocopherol contents in natural spices, total tocopherol contents of 24 different types of spices were quantitatively determined by the Emmerie-Engel colorimetric method. Tarragon, Black pepper, Red pepper, Basil and Allspice were found to have more than 100mg% of tocopherol contents, whereas Sage, Rosemary, Cinnamon, Turmeric and Bay leaves were found to be comparatively lower in tocopherol contents (below 100mg%). The tocopherol contents in Seed Spice such as Caraway, Coriander, Anise seed, Cumin seed, Mace (Aril), Fennel seed, Poppy seed, Celery

View full abstract