Abstract
Growth of, Esch. coli K 12 was retarded with 100 mM ascorbic acid or 10 mM triose reductone. The retarding effect was enhanced with Cu2+and the complete retardation was observed with less amount of them, 10mM ascorbic acid or 1 mM triose reductone, in the presence of 1mM CuSO4. Similar effect was observed for Esch. coli C 600, Esch. coli B, Esch. coli 15 as well as Bac. subtilis 168M. On the other hand, with 1 to 10mM ascorbyl-3-phosphate the growth of Bac. subtilis 168M was promoted. In addition, contrary to ascorbic acid, the promoting ability was counteracted with Cu2+. However, the effect was seldom observed for Esch. coli K 12.