Abstract
To standardize the technique for evaluating food protein with rat, the factors which influence urea nitrogen in blood were investigated. Rats weighing about 100g were fed 7g of 10 and 5% food protein diets in 2 hour daily feeding throughout 12 day feeding Period. Four and 2 hours after the last feeding of 10 and 5% protein diet respectively, blood Plasma was taken and urea nitrogen concentration was determined by colorimetry. Fourtynine foodstuffs and 31 foodstuff mixtures according to the daily menu were tested.
Urea nitrogen concentration of blood was inversely correlated with PER of the corresponding foodstuffs and their mixtures. (10% foodstuff protein diet: r=-0.902 (ρ<0.01), n=29, 5% foodstuff protein diet: r=-0.829 (ρ<0.01), n=13, 10% mixed foodstuff protein diet: r=-0.902 (ρ<0.01), n=17, 5% mixed foodstuff protein diet: r=-0.857 (ρ<0.01), n=12).
