Abstract
Quantitative analysis of free adenine in soybean was made by the following procedures. The defatted soybean powder was extracted with H2O at 4°C for 48 hr, after centrifugation at 3, 000rpm for 20 min. The supernatant fluid was deproteinized with PCA (final concentration, 5%). The deproteinized supernatant was neutralized with KOH and submitted to the ion-exchange chromatography (AG 50W-X8, 1X25cm). The adenine fraction was further applied to the high-performance liquid chromatography (Shim-pack CLC-ODS, 0.15mX6.0 ∅) equipped with UV-VIS spectrophotometric detector. Free adenine content was evaluated from proportion of peak area to that of standard material. Average value of free adenine content in raw soybean was 0.05%.
