Effects of Dzetary Fat on the Reduced Vitamin A Storage and Increased Lipid Peroxide Formation in the Liver of Rats by Combined Administration of PCB and DDT
1985 Volume 38 Issue 4 Pages 273-278
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1985 Volume 38 Issue 4 Pages 273-278
Effects of quality (soybean oil and lard) and quantity (5% and 25%) of fat in the diets on the reduced vitamin A content and increased lipid peroxide formation and cytochrome P-450 induction in the liver were examined with young rats given 0.025% polychlorinated biphenyls (PCB) and 0.01% p, p′-dichlorodiphenyltrichloroethane (DDT).
The results obtained after 10 days of the treatment were compared with the controls. 1. The combined PCB and DDT dose markedly suppressed the growth of rats given the diet containing 25% soybean oil.
2. The reduced vitamin A content in the liver induced by the combined administration of PCB and DDT was not affected by the quality and quantity of dietary fat.
3. Hepatic lipid peroxide formation by the combinatian of PCB and DDT was greater in the group of 25% soybean oil than those in other groups. The result suggests that the enhancement of lipid peraxidation may play a role in the growth retardation. The vitamin E contents in the liver of the 25% fat groups were low as compared to the 5% fat graups, and glutathione peroxidase activity in the liver was significantly lowered by the combined administration of PCB and DDT.
4. An extent of induction of cytochrome P-450 in microsome of the liver by PCB and DDT varied by the quality and quantity of dietary fat. Especially, the induction in the soybean oil groups was higher than those in the lard groups.
5. These results suggest that toxic effects of the combined administration of PCB and DDT are affected by the conditions of the dietary fats.
