Abstract
The effects of intake of a small amount of 4G-β-D-galactosylsucrose (lactosucrose, LS) intake on fecal flora and properties were studied in eight healthy volunteers, who consumed a mixed free-choice diet (control diet), or the same diet with 1g LS/day, 2g LS/day or 3g LS/day. During the intake of LS, bifidobacteria were increased significantly, and lecithinase-positive clostridia including C. perfringens and fecal ammonia were decreased significantly. Fecal concentrations of sulfide showed a significant decrease during intake of 2g LS/day or 3g LS/day. No significant change was observed in water content or stool weight, but a slight change occurred in the fecal pH value during intake of 3g LS/ day. These results indicate that the minimum effective dose of LS in healthy adults is 1-2g/day.