Abstract
A study was undertaken to elucidate the extractabilities of true proteins from grasses using new protein extractants, i. e., 0.05 or 0.1N NaOH solution containing 80%-, 60%-, 40%-, 20%- and 0% acetone. The materials for extraction of true proteins were prepared from fresh grasses by treatment with cold acetone. The highest extractability of true proteins for each material was obtained by successive treatment with the above protein extractants. The values obtained were: spinach 84%, white clover 87%, Japanese radish leaf 91%, water hyacinth 96%, bracken 59%. True proteins of spinach and Japanese radish leaf were partly hydrolyzed by these treatments, and hence their practical yields were 69% and 65%, respectively.
