Abstract
Crude extracts of black matpe showed ACE inhibitory activity. The Sephadex G-100 gel filtration patterns of these extracts indicated that the ACE inhibitory activity was present in the low-molecular-weight fraction. The Sephadex G-15 gel filtration patterns of the active fraction obtained from the Sephadex G-100 column indicated that the molecular weight of the inhibitors was about 400-800. The active fractions obtained from the Sephadex G-15 column were purified by HPLC using an ODS column. The active fractions were not absorbed, and were eluted with 0.05% trifluoroacetic acid. The ACE inhibitory activity of seeds germinated for 6 days was 40% of that of the black matpe extracts. Oral and intraperitoneal administration of a pepsin hydrolysate of the black matpe extract decreased the blood pressure of SHR rats significantly.
