Abstract
We examined the possible chromosomal damaging effect of oxidized oil on mouse bone marrow. The oxidized oils employed were oxidized methyl linoleate (PV4, 500meq/kg) and oxidized methyl linolenate (PV 3, 500meq/kg). Male ICR mice (4 or 6 weeks old) were given various daily doses of either oxidized methyl linoleate (up to 150mg/mouse) or oxidized methyl linolenate (up to 100mg/mouse) by intragastric gavage for 12 days. During the experimental period, peripheral blood was taken every few days for micronucleus assay, which can reveal chromosomal damage in bone marrow. Treatment with the oxidized oil at any dose did not induce chromosomal damage at any time point. Concentrations of vitamin E and glutathione in the bone marrow or liver were decreased only slightly by the treatment, indicating slight induction of oxidative stress by the treatment. The apparent lack of any chromosomal damage by the oxidized oil in the bone marrow is discussed.
