2002 Volume 55 Issue 6 Pages 331-337
Chlorella vulgaris strain CK22 was grown heterotrophically in a pilot-scale fermentor, then docosahexaenoic acid (DHA, 2mL/L) was added to the culture in the stationary phase of growth. The exogenous DHA was taken up by the cells, and accumulated in neutral lipids, glycolipids, and phospholipids. The contents of total lipids and DHA in the cells reached 18.4 and 7.6%, respectively. The glycolipid of the DHA-fortified C. vulgaris strain CK22 consisted of monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and sulfoquinovosyldiacylglycerol. The phospholipid of the cells consisted of phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, and so on. All of the glycolipids and phospholipids fractions contained supplemental DHA among their total fatty acids. The DHA content of the cells showed a tendency to decrease slightly during 6 months of storage at 40°C. Then the DHA in the cells gave little increase of POV (peroxide value) in the cellular lipids.
