異なった2種類の処置をほどこしたチタンプレート表面における骨芽細胞様細胞の付着と増殖および分化に関する研究

抄録

Many morphological studies of cell attachment or surface interaction between titanium and host cells have been carried out. However, these studies did not examine mRNA expression of the cells. Therefore, this study utilized the marker enzymes of cells and the expression of mRNAs in order to evaluate the initial attachment of osteoblastic cells on titanium plates which have different surface micromorphologies.
In this study, the in vitro early cellular responses of osteoblast-like cells (MC3T3-E1) were observed on titanium surfaces with different micromorphologies. Titanium plates were prepared with either a rough or a smooth surface morphology, and the circumference of the titanium plates was covered by a silicone tube for cultivating a fixed number of cells. Alkaline phosphatase (ALP) and lactic dehydrogenase (LDH) were used as marker enzymes. The mRNA expressions of ALP and osteopontine (OPN) were also investigated using reverse transcription-polymerase chain reaction (RT-PCR) analysis.
Significantly higher levels of cellular attachment were found on the rough surface than on the smooth one. In addition, ALP and LDH activity of cultured cells on the rough-surface titanium plates were higher than those on the smooth-surface ones.The mRNA expressions of ALP and OPN of cultured cells on the rough-surface titanium plates were enhanced compared with those on the smooth surface plates.
These findings demonstrate that surface roughness alters osteoblast-like cell proliferation, differentiation, and matrix production in vitro. The results also suggest that surface micromorphologies influence the cellular responses of osteoblast-like cells, although further quantitative analyses are required.