H₂O₂水熱酸化処理とFGF-2によるチタンの表面機能化のin vitroにおける評価

抄録

The present study aimed to demonstrate the influence of surface treatment of fibroblast growth factor (FGF)-2-loaded TiO₂ film by H₂O₂ hydrothermal treatment on in vitro bone bioactivity.

The results suggested that H₂O₂ hydrothermal treatment resulted in TiO₂ films with a lower contact angle and higher hydrophilicity. Early cell adhesion assay by a scanning electron microscope (SEM) suggested that the FGF-2-loaded surface had a high cell density and a significantly higher cell average adhesion area, as well as marked cell extension when compared with the non-FGF-2-loaded surface. MC3T3-E1 osteoblast-like cell proliferation tended to increase with time, with particularly high cell proliferation observed on day 7 and day 14. The alkaline phosphatase (ALP) activity was significantly higher in the FGF-2-loaded group when compared with the nonFGF-2-loaded group.

In conclusion, the results of this study showed that H₂O₂ hydrothermal surface treatment of FGF-2-loaded TiO₂ films can increase early cell adhesion, proliferation and bone differentiation, and contribute to functionalization of the titanium surface, early bone-titanium implant integration and long-term stabilization.