Abstract
Calcium and phosphate contents, and alkaline and acid phosphatase (ALPase and ACPase) activities in serum of Wistar Strain rat fed with low Ca and Be diets were examined.
Fourty 4-week-old male rats of the Wistar Strain with initial body weight of 50-60 gr were used. Those animals were divided into 4 groups and were respectively fed with a normal well balanced diet (control group), a low Ca diet (low Ca group), a Be diet (Be group) and a Be·Ca diet (Be·Ca group) for 4 weeks.
At the end of the experiment period, serum were obtained from the carotid artery of all rats. Using this serum, we measured Ca and P with atomic absorption spectrophotomotry, ALPase and ACPase activity with Kind and King's method, and protein with Lowry's method.
The conditions of growth and development were worse in the order of the Be·Ca, the Low Ca and the Be groups than those in the control group.
Serum calcium decreased in the low Ca and Be groups, but in the Be·Ca group, it was similar to that in the control group.
Serum P decreased in low Ca group and furthermore decreased in the Be group. It also decreased in the Be·Ca group, but its value was similar to that in the low Ca group.
ALPase activity increased remarkably in the low Ca group. This acceleration was inhibited in the Be and the Be·Ca groups.
From these facts, it was suggested that low Ca diets accelerated ALPase activity and beryllium inhibited phosphate metabolism.
