Determination of cephalothin and its metabolite, desacetylcephalothin, in human serum employing high performance liquid chromatography

Abstract

Cephalothin (CET) and its metabolite, desacetylcephalothin (DACET) were determined in human serum by using high performance liquid chromatography (HPLC), and the results showed good correlation with those so far obtained from bioassay.
In addition, CET and DACET serum levels after CET administration were determined in four patients undergoing hemodialysis for renal failure. The results showed that the levels were higher and the individual variation were greater in these patients than in normal, healthy sub-jects, in which a tendency for DACET to decrease with time was observed.