“Inteban ointment”contained a non steroidal anti-inflammatory agent of indomethacin, was used to 36 patients of mandibular arthrosis without any other treatment.
The application method of the ointment was that 5 to 8g of them for one day were divided to 4 times, and rubbed in tempolo-mandibular joint region after meals and before sleep.
The evaluation of the clinical efficacy was judged by score method that each symptoms of mandibular arthrosis were given the standardized score by the severity, compared and analysed with statistically the score of first day, 7th day and 14th day from the start of application.
The results obtained were as follows:
1. Average score of the first day 10.58 was improved to 6.39 at 14th day, showing a significant difference (p<0.001, t-test) .
2. The symptoms show the remarkable improvements were oppressive pain on tempolomandibular joint region, and spontaneous pain at mandibular movement.
3. Side-effect was not recognized in this series.

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The antibiotics studied were the cefems. The MIC for ceftezole (CTZ), cefazolin (CEZ), cephalothin (CET), cephaloridine (CER) and cephapirin (CEPR) were determined by incubation in assay cultures for limited periods of time using Staphylococcus aureus 209P JC-l as the test organism and heart infusion broth (HI broth, Difco) as the assay medium. Bacterial growth was measured using a biorecorder (Toyo Kagaku) that was designed to record automatically the optical density (O.D.) of turbid broth in twelve L-shaped test tubes (L-tubes) continuously or at designated points during incubation with constant shaking at a prescribed temperature. Eleven L-tubes containing 10 ml aliquots of HI broth to which were added serial ten-fold dilutions of antibiotic (100 to 0.10μg/ml) and one control L-tube containing antibiotic-free HI broth were inoculated with 10⁶ test organisms per ml and incubated at 37°C with constant shaking. At the end of 1 and 3 h of incubation, 10μl of broth from each tube were transferred to tubes con-taining 10 ml of fresh broth and were incubated at 37°C with shaking in the biorecorder. The time required for the O.D. to reach T-0.10 (equivalent to 108 organisms per ml) was determined by continuous readings at 5 min intervals. The difference in time for reaching an O.D, of T-0.10 between the antibiotic culture and the control was expressed as growth retardation time.
In summary, exposure of the test microorganism to 1.56μg/ml of CER for 1 h resulted in a delay in bacterial growth of up to 6h, and exposure to an increased concentration of 6.25μg/ml led to a delay of more than 15h. However, an increase in antibiotic concentration from 1.56 to 6.25μg/ml for the other four cefems resulted in prolonged growth lags that did not exceed 5h.
The organism showed a delay in growth by more than 15h after exposure to CER for a period of 3h, even with an antibiotic concentration as low as 1.56μg/ml resulted in a delay in growth by more than 15h. In addition, exposure to a comparable concentration of CEPR resulted in a delay of 12h. For the other three cefems, a three-hour exposure at concentrations of 1.56μg/ml led to growth lags of 5.4 to 6.6h, and exposures to increased concentrations of 6.25μg/ml re-sulted in a delay by more than 13, 7.2 and 7.6h with CET, CTZ and CEZ respectively. Analysis demonstrated no significant differences in lag time with exposure to 1.56μg/ml.
Significantly, the results indicate that there are marked pharmacodynamic differences among these cefems that are currently used in daily clinical practice.

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Myoactin C, which was used for alveolar pyorrhea, is composed of ATP, L-tyrosine and L-cysteine, as fix combination drugs.
The author observed that ATP increased the tone of smooth muscle at a low concentration, and decreased it as a high concentration on the mechanical activity of the smooth muscle (portal vein), but the effects of Myoactin C on the smooth muscle can be considered that they relate closely with L-tyrosine, +L-cysteine, +ketoglutaric acid.

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In the dental treatment of hypertensive patients, we applied nifedipine, which reliable and mild effects are expectable, and obtained good results.
The subjects used are 10 hypertensive patients categorized as the stage I and II of WHO classification who visited our hospital for tooth extraction. The ages of patients ranged from 54 to 76 years old.
Local anesthesia with 2% lidocaine containing 1/80, 000 epinephrine was applied 20 min after sublingual administration of 10mg of nifedipine. Then, 10 min later, blood pressure, mean blood pressure and pulse rate were measured.
Both systolic and diastolic pressures started to decline 5 min after the administration of nifedipine, reached the minimum level after 20 min and stayed at that level until 60 min after the administration.
Pressor effect due to the local anesthesia and tooth extraction was mild, and hemodynamics was kept stable. Also, neither dizziness nor body sway was observed.

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In this study, 6 injection antibiotics; cephaloridine (CER), cefazolin (CEZ), ceftezole (CTZ), cefoperazone (CPZ), cephalothin (CET) and cephapirin (CEPR) and 2 oral antibiotics; cephalexin (CEX) and cefadroxil (CDX), were used. Four strains of Staphylococcus aureus were used as test organisms. Heart infusion broth (HI broth—Difco) was used as a medium for the test. A biorecorder (Toyo Kagaku Co., Ltd.) was used for measurement. The amount of bacteria added was 5×10⁵ cells/ml, and the biorecorder was set to record the optical density at the moment of the growth of the added bacteria up to 5×10⁷ cells/ml.
Study 1: By determination of the effect of cephalosporinase (Chemical Credentials Research) containing 290 units of activity per mg, it was confirmed that 8 cefems used in this study lose their antimicrobial activity by addition of cephalosporinase (290 units/ml) . Consequently, it was designed in this study to add 290 units of cephalosporinase per ml of broth.
Study 2: Six cefems for injection and Staph. aureus 209P JC-1 were left in contact with shaking for 3 h at 37°C. Cephalosporinase was then added to the broth, and shaking was continued during the culture, and the delayed growth time was determined. At the concentration of 0.78μg/ml, the delayed growth time was longest with CER (14.0h), followed by CEPR (13.6h), CTZ (12.1h), CPZ (9.7 h), CET (9.4h) and CEZ (7.2h) . At 1.56μg/ml, it was longest with CEPR (14.3h), followed by CER (14.2h), CTZ (12.5h), CPZ (10.3h), CET (9.7h) and CEZ (7.3h) . At 3.13μg/ml, it was longest with CEPR or CFR (14.4h), followed by CTZ (12.6h), CPZ (12.4h), CET (9.9h) and CEZ (7.4h) . At 6.25μg/ml, it descended in the order of CEPR (14.6h) >CER (14.3h) >CTZ (12.9h) >CPZ (12.5h) >GET (10.4h) >CEZ (7.5h) . For each cefem the delayed growth time showed dose-dependent prolongation with an increase in the concentration.
Study 3: Limiting the exposure time to 3h, we studied 4 strains of Staph. aureus with GER. It was found that there were individual variations in the delayed growth time among these strains of organisms. In addition, this study also confirmed that the delayed growth time was prolonged dose-dependently with an increase in the concentration.
Study 4: The organisms were exposed to CER for 2, 3 or 4 h. The delayed growth time was found to be dependent upon the exposure time.
Study 5: Using 2 oral antibiotics, CEX and CDX, and limiting the exposure time to 3.5h, we determined the delayed growth time. As a result, it was found that the delayed growth time was prolonged with CDX at the concentration of 1.56-6.25μg/ml.
As regards cefems for injection use, although the delayed growth time varied individually, a time-dependent relationship was noted between the delayed growth time and the time of exposure to antibiotics. Although time-dependence was noted, prolongation of the delayed growth time was short at concentrations above a certain level. From this point of view, it was considered that cefems for injection use would be more effective even at low concentrations when the exposure time was longer. Especially CER, CEPR and CTZ, resulted in long prolongation of the delayed growth time even at low concentrations, and their activity depended little upon the method of administration. Consequently, these antibiotics were confirmed to be useful in this in vitro ex-periment.

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A multi-clinic double-blind study was conducted to examine the analgesic efficacy and safety of Suprofen, a new non-steroidal anti-inflammatory agent, in comparison with indomethacin after tooth extraction. Patients were administered either Suprofen 200mg or indomethacin 25mg.
1. Statistically analyzed cases were 101 with Suprofen group and 106 with indomethacin group, 207 as total. Drop out and excluded cases were 5 with Suprofen group and 7 with indomethacin group.
2. In global improvement rating of a pain and overall clinical evaluation, Suprofen group was significantly superior to indomethacin group (ρ<0.01) .
3. In time till appearance of effect, Suprofen group was significantly faster to indomethacin group (ρ<0.01) .
4. Side effects were reported 7 cases in Suprofen group and 5 cases in indomethacin group, and no significantly differences were shown between the two groups.
Summarizing the above results, it can be concluded that Suprofen is a useful drug after tooth extraction and that analgesic efficiency and safety on Suprofen administration were better than indomethacin administration.

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A multi-center double-blind study in comparison with aspirin was conducted to evaluate the effectiveness, safety and usefulness of Suprofen (TO-177), a new non-steroidal analgesic/anti inflammatory agent, in tooth extraction of mandibular impacted wisdom tooth.
Patients were treated with Suprofen (SP) 100mg three times a day or aspirin (AP) 500mg three times a day.
The results were obtained as follows.
1) Total treated cases were 272 (SP: 133 cases, AP: 139 cases) . 4 cases were excluded.
2) According to the clinical efficacy, the ratio of“good or better”was 74.4% with SP group and 61.2% with AP group. There was a significant difference between two groups (p<0.001) .
3) Side effects were reported 3 cases (2.3%) SP group and 10 cases (5.1%) AP group.
4) In the general utility rating, the ratio of“useful or better”was 72.7% with SP group and 56.7% with AP group. There was a significant difference between two groups (p<0.001) .
The results indicate that Suprofen is a safe and useful anti inflammatory analgesic agent.

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Cephalothin (CET) and its metabolite, desacetylcephalothin (DACET) were determined in human serum by using high performance liquid chromatography (HPLC), and the results showed good correlation with those so far obtained from bioassay.
In addition, CET and DACET serum levels after CET administration were determined in four patients undergoing hemodialysis for renal failure. The results showed that the levels were higher and the individual variation were greater in these patients than in normal, healthy sub-jects, in which a tendency for DACET to decrease with time was observed.

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